CTCCTGGTCC-3 (BamHI internet sites are underlined). The PCR goods were digested by
CTCCTGGTCC-3 (BamHI websites are underlined). The PCR solutions had been digested by BamHI, and inserted in to the BglII web site of either pBridge or pBridge-AGG1, generating pBridge-2-AGB1 or pBridge-AGG1-AGB1,…