S associated together with the terms “transcription,” “positive regulation of gene expression,” “response to nutrient levels,” and “autophagy.” Down-regulated phosphorylation occurred on proteins associated together with the terms “cell cycle,” “M phase,” and “site of polarized development,” and these terms have been far more substantially enriched at the 3-h time point, suggesting that down-regulation of phosphorylation could have resulted from lowered cell division. To recognize proteins with equivalent regulation, we clustered quantified phosphorylation sites according to their temporalMolecular Cellular Proteomics 13.Phosphorylation and Ubiquitylation Dynamics in TOR SignalingA0.Fraction of peptidesBNumber phosphorylation web sites 0.15 0.ten 0.05 0 7000 6000 5000 4000 3000 2000 1000 0 6339 unmodified unregulated regulatedn =371 sites918 sites5002 three 4 Cluster–2 0 2 Log2 SILAC ratio (1h/Ctrl)C1.0 0.5 0.0 -0.five -1.0 1.0 0.5 0.0 -0.five -1.0 1.0 0.5 0.0 -0.five -1.0 1.0 0.5 0.0 -0.5 -1.0 1.0 0.five 0.0 -0.5 -1.0 1.0 0.5 0.0 -0.five -1.0 0 1 Time (h) 1 0.8 0.six 0.four 0.two 0 3 ClusterDnuclear telomere cap complicated mitotic anaphase B RNA polymerase II core binding snoRNA transcription from an RNA polymerase II promoter microtubule bundle formation aspartate kinase activity methylenetetrahydrofolate reductase (NADPH) activity phosphorylase activity kinetochore microtubule nuclear microtubule transcription from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter methionine metabolic method telomere maintenance via telomerase glycogen phosphorylase activity plus-end-directed microtubule motor activity fungal-type cell wall biogenesis telomerase inhibitor activity optimistic regulation of gene expression telomere capping regulation of telomere maintenance through telomerase transcriptionally active chromatin mitotic spindle stabilization nuclear SCF ubiquitin ligase complicated triplex DNA binding spindle midzone assembly regulation of histone H3-K4 methylation unfavorable regulation of telomerase activity regulation of transcription initiation from RNA polymerase II promoter constructive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues nuclear matrix spindle pole body separation regulation of chromatin silencing at telomere astral microtubule protein ubiquitination involved in ubiquitin-dependent protein catabolic process damaging regulation of Rho protein signal transduction Rho GTPase activator activity G1/S transition of mitotic cell cycle signal transduction GTPase activator activity good regulation of GTPase activity phosphatidylinositol-3-phosphate binding cell communication phosphatidylinositol binding mitotic cell cycle spindle assembly checkpoint ubiquitin-protein ligase activity mitochondrial inner membrane cell cortex trans-Golgi network protein targeting to vacuole involved in ubiquitin-dependent protein catabolic procedure via the multivesicular body sorting pathway Golgi apparatus portion Golgi to endosome transport intracellular transcription elongation from RNA polymerase II promoter transferase activity, transferring phosphorus-containing groups protein serine/threonine kinase activity protein kinase activity protein phosphorylation establishment of cell polarity extrachromosomal circular DNA localization involved in cell aging cytoskeletal regulatory protein binding constructive regulation of formin-nucleated actin cable assembly intermediate filament cell shape checkpoint single-stranded DNA particular endodeoxyribonuclease activity.Price of 945459-80-3 936637-97-7 web PMID:23341580