E and kelampayoside Anegative mode, the [M – H]-, [M + HCOOH – H]- and [M + 2H two O – H] – ions had been simultaneously observed. The MS/MS spectrum showed a important fragment ion [C11H18O9 – H]- ion at m/z 293 which was attributed for the loss of a C1 unit, in the similar time, other ions m/z 233, m/z149 and m/z131 have been observed [Figure 3].The ESIMSn of swerosideThe [M – H]-, [M + 2H2O – H]- and [M + HCOOH – H]- ions had been shown in the negative mode. Inside the MS/MS spectrum analysis of [M + HCOOH – H]- ion, the ion triggered by the losses of glucose as well as other units successively were observed [Figure 4]. In frequent with sweroside, log anin g ave the [M – H]-, [M + 2H2O – H]- and [M + HCOOH – H]- ions inside the unfavorable mode. The MS/MS spectrum of m/z 435 revealed a radical aglycone ion [M – glu – H]- at m/z 227 because the base peak, resulting from the loss of glucose (162 Da). Within the additional MSn analysis of [Mglu – H]- ion of loganin, the clear [MgluH2O – H]- ion at m/z 209 was observed, meanwhile, other ions m/z 127 and m/z 101 had been observed [Figure 5]. In the constructive mode, the ESIMSn of loganin was constant with literature.[28]T he 3,4dimethoxyphenol Dapiofuranosyl (1 six)Dglucopyranoside was located in the genus Nauclea for the initial time and kelampayoside A was isolated from Nauclea officinalis for the initial time in our lab, respectively.[23] The structures from the two compounds had been pretty comparable to every other except for the CH3 at the C5 position. The analogies of structures indicated the similarities of their MS n fragmentation behaviors. In their ESIMS spectra, the [M + Na] + ion have been obtained within the positive mode, though in thePharmacognosy Magazine | July-September 2014 | Vol ten | IssueZhu, et al.: Qualitative and quantitative analysis of constituents in Danmu injectionTable 2: HPLCDADESI/MS identification benefits of 25 chemical compositions in Danmu injectionPeak no. D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 D13 D14 D15 D16 D17 D18 D19 D20 D21 D22 D23 D24 D25 Retention time (tR, min) 19.2 20.1 21.7 24.three 24.6 27.six 28.1 28.three 29.3 30.2 31.3 32.3 32.6 34.7 36.two 37.3 38.9 43.0 46.0 46.four 54.six 57.2 63.five 57.two 70.5 UV max (nm) 259.6, 292.eight 252.five, 291.six 218.3, 327.three 234.8 228.1, 279.7 214.7, 329.7 241.8 204.two, 271.4 218.three, 327.3 224.two, 273.3 241.five 225.2, 274.1 246.six 224.3, 273.4 224.2, 272.six 208.1,243.two 224.2, 271.four 241.eight, 327.3 243.0, 327.three 226.two 226.eight 226.three 224.two 226.five 226.five Characteristic ion fragments (m/z) 153[M-H]-; MS2:109 461[M-H]-; MS2:293,167 353[M-H]-; MS2:191 (100),179; MS3:173,135 375[M-H]-; MS2:213,169 493[M+HCOOH-H]-,483[M+2H2O-H]-,447[M -H]-(100); MS2:293 (100),233,149,131,125 353[M-H]-; MS2:191 (100),179; MS3:173,135 435[M+HCOOH-H]-(one hundred),425[M+2H2O-H]-; MS2:388,227 (100); MS3:209,177,127,101 523[M+HCOOH-H]-,513[M+2H2O-H]-(one hundred),477[M-H]-; MS2:293 (100),233,149,131,125 353[M-H]-; MS2:191 (100),179,173; MS3:135 519[M+H]+; MS2:357 (one hundred),339; MS3:321,309,160 431[M+HCOOH-H]-(one hundred),403[M+H2O-H]-; MS2:393,231,213,167,101 519[M+H]+; MS2:357 (one hundred),339;MS3:321,309,160 403[M+HCOOH-H]- (one hundred),393[M+2H2O-H]-; MS2:357 (100),195,179,125 681[M+H]+; MS2:519,357 (one hundred);MS3:339,321,309,160 519[M+H]+; MS2:501,357 (one hundred),339; MS3:321,309,160 531[M+H]+; MS2:511,369,349,299 489[M+H]+; MS2:471,327 (100),309; MS3:309,186,160 513[M+H]+; MS2:351,333,281; MS3:263,185,158 513[M+H]+; MS2:351,333,281; MS3:263,185,158 514[M+H]+; MS2:353 (100),283,171,187 515[M+H]+; MS2:353 (one hundred),335,317,283; MS3:283,265,160,132 515[M+H]+, MS2:353 (one hundred),335,317,283; MS3:283,265,160,132 543[M+H].Benzene-1,3,5-tricarbaldehyde Order 937048-76-5 web PMID:24367939
